Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro.

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May 20, 2021 0 Comments

Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro.

The aim of this study was to assess the factors, mechanisms and the differences between periodontal ligament (PDL) cells and denta l follicle (DF) progenitor cells towards the osteoblastic/cementoblastic differentiation and to investigate the effects of BMP-7 on developmental (DF) and mature tissue-derived (PDL) cells, respectively. Primary cell culture of PDL cells and DF progenitor cells was performed. Osteogenic differentiation was evaluated using von Kossa, Alizarin Red S and immunohistochemistry staining of osteocalcin.
Gene expression pattern was evaluated via real-time PCR. A series of CD surface marks were tested using flow cytometry and fluorescence-activated cell-sorting analysis was performed. Real-time RT-PCR demonstrated similar gene expression pattern of PDL cells and DF progenitor cells: the expression of OPN and OCN significantly was elevated when incubated with osteogenic components, Runx2 was unaffected, and Osteorix was hardly expressed whether in basic medium or induction medium. In addition, BMP-7 induced osteoblast/cementoblast differentiation of PDLSCs and DF progenitor cells in a dose- and time-dependent manner, as reflected by enhanced Runx2 and (OCN) mRNA transcript expression. BMP-7 triggers PDL cells and DF progenitor cells to differentiate towards an osteoblast/cementoblast phenotype.

 

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Description: DNA Fragmentation Assay Kit for use in the research laboratory

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1-CSB-EP006737HU
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  • EUR 560.00
  • EUR 873.00
  • EUR 262.00
  • 100ug
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  • 1MG
  • 200ug
  • 500ug
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Description: Recombinant Human DNA fragmentation factor subunit alpha(DFFA) expressed in E.coli

Recombinant DNA Fragmentation Factor Subunit Alpha (DFFa)

4-RPL426Hu01
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Description: Recombinant Human DNA Fragmentation Factor Subunit Alpha expressed in: E.coli

Recombinant DNA Fragmentation Factor Subunit Alpha (DFFa)

4-RPL426Mu01
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Description: Recombinant Mouse DNA Fragmentation Factor Subunit Alpha expressed in: E.coli

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  • 100 ug
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Mouse DNA Fragmentation Factor Subunit Alpha (DFFa) Protein

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DFFA DNA Fragmentation Factor Subunit Alpha Human Recombinant Protein

PROTO00273 Regular: 25ug
EUR 317
Description: DFFA Human Recombinant fused with 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 351 amino acids (1- 331 a.a.) and having a molecular mass of 38.7kDa.;The DFFA is purified by proprietary chromatographic techniques.

DNA Fragmentation Factor Subunit Alpha (DFFa) Polyclonal Antibody (Human)

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Description: A Rabbit polyclonal antibody against Human DNA Fragmentation Factor Subunit Alpha (DFFa)

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Description: A Rabbit polyclonal antibody against Human DNA Fragmentation Factor Subunit Alpha (DFFa). This antibody is labeled with APC.

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  • 10ml
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Description: A Rabbit polyclonal antibody against Human DNA Fragmentation Factor Subunit Alpha (DFFa). This antibody is labeled with Biotin.

DNA Fragmentation Factor Subunit Alpha (DFFa) Polyclonal Antibody (Human), Cy3

4-PAL426Hu01-Cy3
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  • EUR 4733.00
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  • EUR 263.00
  • 100ul
  • 10ml
  • 1ml
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Description: A Rabbit polyclonal antibody against Human DNA Fragmentation Factor Subunit Alpha (DFFa). This antibody is labeled with Cy3.

DNA Fragmentation Factor Subunit Alpha (DFFa) Polyclonal Antibody (Human), FITC

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Description: A Rabbit polyclonal antibody against Human DNA Fragmentation Factor Subunit Alpha (DFFa). This antibody is labeled with FITC.

DNA Fragmentation Factor Subunit Alpha (DFFa) Polyclonal Antibody (Human), HRP

4-PAL426Hu01-HRP
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  • 100ul
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Description: A Rabbit polyclonal antibody against Human DNA Fragmentation Factor Subunit Alpha (DFFa). This antibody is labeled with HRP.

DNA Fragmentation Factor Subunit Alpha (DFFa) Polyclonal Antibody (Human), PE

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  • EUR 313.00
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  • EUR 202.00
  • 100ul
  • 10ml
  • 1ml
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Description: A Rabbit polyclonal antibody against Human DNA Fragmentation Factor Subunit Alpha (DFFa). This antibody is labeled with PE.

DNA Fragmentation Factor Subunit Alpha (DFFa) Polyclonal Antibody (Human, Mouse)

4-PAL426Mu01
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  • EUR 337.00
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  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse DNA Fragmentation Factor Subunit Alpha (DFFa)

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EUR 875.05
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EUR 860.55
Description: Ask the seller for details

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EUR 635
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DNA Fragmentation Factor Subunit Alpha (DFFa) Polyclonal Antibody (Human), APC-Cy7

4-PAL426Hu01-APC-Cy7
  • EUR 614.00
  • EUR 7042.00
  • EUR 1858.00
  • EUR 821.00
  • EUR 337.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human DNA Fragmentation Factor Subunit Alpha (DFFa). This antibody is labeled with APC-Cy7.

DNA Fragmentation Factor Subunit Alpha (DFFa) Polyclonal Antibody (Human, Mouse), APC

4-PAL426Mu01-APC
  • EUR 374.00
  • EUR 3689.00
  • EUR 1016.00
  • EUR 481.00
  • EUR 232.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse DNA Fragmentation Factor Subunit Alpha (DFFa). This antibody is labeled with APC.

DNA Fragmentation Factor Subunit Alpha (DFFa) Polyclonal Antibody (Human, Mouse), Biotinylated

4-PAL426Mu01-Biotin
  • EUR 332.00
  • EUR 2763.00
  • EUR 803.00
  • EUR 411.00
  • EUR 228.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse DNA Fragmentation Factor Subunit Alpha (DFFa). This antibody is labeled with Biotin.

DNA Fragmentation Factor Subunit Alpha (DFFa) Polyclonal Antibody (Human, Mouse), Cy3

4-PAL426Mu01-Cy3
  • EUR 457.00
  • EUR 4877.00
  • EUR 1313.00
  • EUR 600.00
  • EUR 267.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse DNA Fragmentation Factor Subunit Alpha (DFFa). This antibody is labeled with Cy3.

antigenretrieve

[Analysis on clinical and pathological characteristics of 66 patients with stage IV breast cancer].

OBJECTIVE
To explore the clinical and pathological characteristics of stage IV breast cancer and to analyze their relationship with the morbidity and prognosis.
METHODS
The records of 66 patients presenting from January 2008 to December 2014 with stage IV breast cancer were reviewed. All of the patients were women and the median age was 57.5 (31 to 80) years, accounted for 3.01% (66/2 189) among the breast cancer patients treated in the same period. Statistical methods were used to analyze the correlation between clinical and pathological characteristics such as T-stage, N-stage, immunohistochemistry and the morbidity and prognosis of stage IV breast cancer. The influence of patients characteristics to metastasis were compared by χ(2) test. Kaplan-Meier curves were reported for overall survival (OS), and the Log-rank test was used to compare the difference in groups. Cox proportional models were fitted for multivariate analysis.
RESULTS
The median survival time of stage IV breast cancer was 56.0 months and the 5-year survival rate was 40%. To metastasis, the effects of age, subtypes, histological grade, hormone receptor (HR) and human epidermal growth factor receptor 2 (HER2)had no significant statistics differences. It was concluded that the expression of HER2 (P=0.003) and HR (P=0.001) as well as single metastasis (P=0.029) were the influencing factors of the survival by multivariate Cox regression analysis. Primary tumor R0 surgery group and no surgery group had no significant statistics differences of overall survival and the 5-year survival rate (P=0.102).
CONCLUSIONS
Clinical and pathological characteristics have no effect on metastasis. The expression of HER2 and HR as well as single metastasis play important roles in survival.

Cell lines of human oral squamous-cell carcinomas retaining their differentiated phenotype.

Two cell lines from head-and-neck squamous-cell carcinomas (SCC) have been established and characterized. Cell line R105 was derived from a xenografted SCC of the floor of the mouth and cell line T87/rc from a SCC of the epiglottis. Identification of individual cytokeratins 4, 5, 7, 8, 10, 13, 14, 18 and 19 led to the conclusion that both cell types had squamous characteristics and that keratinization occurred in xenografts. Ultrastructurally, junctional complexes were observed in both cell lines. Characteristic marker chromosomes were found and although both cell lines were derived from male patients, the Y chromosome was missing from all examined cells. The basic biological parameters of both cell lines were modal chromosome numbers of 59 (R105) and 60 (T87/rc), a doubling time of 60 (R105) and 45 hr (T87/rc) and a DNA index of 1.54 (R105) and 1.31 (T87/rc).
The tumorigenicity of the 2 cell lines was proved by the ability to form colonies on a plastic substratum, as well as in a soft agar assay. Furthermore, the cells could produce multi-cellular tumour spheroids, and formed tumour nodules after subcutaneous inoculation into nude mice. The R105 tumour cells appeared to be better differentiated than the T87/rc as observed by histology and immuno(histo)chemistry. Both cell lines appear to retain SCC differentiation after being xenografted into nude mice, cultured for more than 40 passages in vitro and thereafter again xenografted into nude mice.

Tissue engineering in urinary bladder: morphological and functional characterization.

BACKGROUND
In the last years, tissue engineering has attracted lots of researchers, in urology too. This is due to the possibility to use this technique in several pathologies’ therapies, which generally require reconstructive surgical solutions. Our work’s aim is to evaluate morphological and functional aspects of cultivated urothelial and detrusorial tissues, both in “monolayer growth” and on scaffolds, in order to understand the chance of using them in reconstructive surgery.
METHODS
Tissue cultures of detrusorial and urothelial cells have been obtained from animals (pigs, rabbits) and men. The urothelial nature of obtained cells has been demonstrated by traditional histological observation (Hematoxylin – Eosin), by immuno-fluorescence assay (specific for cyto-keratins antibodies), by immunohistochemistry techniques (using specific cyto-keratins 7, 17, and 20 antibodies). Detrusorial tissue has been studied by using antibodies specific for alpha-actin.
RESULTS
Urothelial and smooth muscle cells, when isolated and expanded in vitro, keep the typical characteristics of original tissue, as showed by classical histological observation (H-E), immunohistochemistry and immuno-fluorescence assays. These results were positive both in monolayer colonies and on scaffolds. In vitro results are encouraging and they demonstrate that it is possible to obtain in vitro vesical tissue that could have analogous characteristics to the original organ; even though clinical utilisation of this technique must be more investigated, both in vitro and in vivo.

 

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EUR 805
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

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EUR 805
Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity:

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DAB

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DAB Tablet

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DAB Chromogen Concentrate

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EUR 161

DAB Chromogen Concentrate

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Boc-Dab-OH

A-3215.0001 1.0g
EUR 126
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Boc-Dab-OH

A-3215.0005 5.0g
EUR 418
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

Boc-Dab-OH

A-3215.0025 25.0g
EUR 1578
Description: Sum Formula: C9H18N2O4; CAS# [25691-37-6]

H-Dab-OH·HCl

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Fmoc-Dab-OAll·HCl

5-04720 5g Ask for price

Fmoc-Dab-OH·HCl

5-04721 5g Ask for price

Fmoc-Dab-OH·HCl

5-04722 25g Ask for price

Fmoc-Dab-OH

5-04735 1g Ask for price

Fmoc-Dab-OH

5-04736 5g Ask for price

DAB Chromogen Kit

abx097197-1Kit 1 Kit
EUR 272

H-Dab-OH

F-3050.0001 1.0g
EUR 115
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

H-Dab-OH

F-3050.0005 5.0g
EUR 225
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

H-Dab-OH

F-3050.0025 25.0g
EUR 803
Description: Sum Formula: C4H10N2O2; CAS# [1883-09-6]

Z-Dab-OH

C-3705.0001 1.0g
EUR 115
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Z-Dab-OH

C-3705.0005 5.0g
EUR 273
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Z-Dab-OH

C-3705.0025 25.0g
EUR 998
Description: Sum Formula: C12H16N2O4; CAS# [62234-40-6]

Fmoc-Dab-OH

B-2300.0001 1.0g
EUR 151
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7]

Fmoc-Dab-OH

B-2300.0005 5.0g
EUR 515
Description: Sum Formula: C19H20N2O4; CAS# [161420-87-7]

DAB Chromogen, 4ml

NB314D 4 ml
EUR 246

DL-Dab.2HCl

A6727-100000 100 g
EUR 569
Description: DL-Dab.2HCl

DL-Dab.2HCl

A6727-25000 25 g
EUR 309
Description: DL-Dab.2HCl

DL-Dab.2HCl

A6727-5000 5 g
EUR 180
Description: DL-Dab.2HCl

DAB Chromogen Concentrate

ACB030 30 ml
EUR 119

DAB Chromogen Concentrate

ACB060 60 ml
EUR 165

DAB Chromogen Concentrate

ACB125 125 ml
EUR 245

DAB Chromogen Concentrate

ACB500 500 ml
EUR 671

DAB Chromogen Concentrate

ACB999 1000 ml
EUR 1159

DAB Enhancer Solution

ACM030 30 ml
EUR 71

DAB Away Kit

ACP001 500 ml
EUR 98

DAB Differentiating Solution

DDS500 500 ml
EUR 109

DAB Enhancer for ampliying DAB stains in IHC staining, 60ml

NB308 60 ml
EUR 427

DAB Enhancer for ampliying DAB stains in IHC staining, 30ml

NB308-30 30 ml
EUR 299

Sensitive DAB Stain Kit

PW023 5Preps, 5prep
EUR 69.14

H-Dab(Boc)-OH

A-3305.0001 1.0g
EUR 200
Description: Sum Formula: C9H18N2O4; CAS# [10270-94-7]

H-Dab(Boc)-OH

A-3305.0005 5.0g
EUR 696
Description: Sum Formula: C9H18N2O4; CAS# [10270-94-7]

Boc-Dab(Fmoc)-OH

A-3520.0001 1.0g
EUR 225
Description: Sum Formula: C24H28N2O6; CAS# [117106-21-5]

Boc-Dab(Fmoc)-OH

A-3520.0005 5.0g
EUR 780
Description: Sum Formula: C24H28N2O6; CAS# [117106-21-5]

Boc-Dab(Aloc)-OH

A-4125.0001 1.0g
EUR 309
Description: Sum Formula: C13H22N2O6; CAS# [171820-73-8]

Boc-Dab(Aloc)-OH

A-4125.0005 5.0g
EUR 1143
Description: Sum Formula: C13H22N2O6; CAS# [171820-73-8]

Boc-D-Dab-OH

A-4215.0001 1.0g
EUR 418
Description: Sum Formula: C9H18N2O4; CAS# [80445-78-9]

Boc-D-Dab-OH

A-4215.0005 5.0g
EUR 1554
Description: Sum Formula: C9H18N2O4; CAS# [80445-78-9]

Boc-Dab-OtBu · HCl

A-4415.0001 1.0g
EUR 309
Description: Sum Formula: C13H26N2O4·HCl; CAS# [190447-69-9] net

Boc-Dab-OtBu · HCl

A-4415.0005 5.0g
EUR 1143
Description: Sum Formula: C13H26N2O4·HCl; CAS# [190447-69-9] net

H-Dab(Z)-OH

5-04707 5g Ask for price

H-Dab(Z)-OH

5-04708 25g Ask for price

Boc-Dab(Boc)-OH·DCHA

5-04713 1g Ask for price

Boc-Dab(Boc)-OH·DCHA

5-04714 5g Ask for price

Boc-Dab(Fmoc)-OH

5-04715 5g Ask for price

Boc-Dab(Fmoc)-OH

5-04716 25g Ask for price

Boc-Dab(Z)-OH·DCHA

5-04717 5g Ask for price

Boc-Dab(Z)-OH·DCHA

5-04718 25g Ask for price

Fmoc-Dab(Ac)-OH

5-04723 5g Ask for price

Fmoc-Dab(Ac)-OH

5-04724 25g Ask for price

Fmoc-Dab(Boc)-OH

5-04725 5g Ask for price

Fmoc-Dab(Boc)-OH

5-04726 25g Ask for price

Fmoc-Dab(Fmoc)-OH

5-04729 1g Ask for price

Fmoc-Dab(Fmoc)-OH

5-04730 5g Ask for price

Fmoc-Dab(MMt)-OH

5-04731 5g Ask for price

Fmoc-Dab(MMt)-OH

5-04732 25g Ask for price

Fmoc-Dab(Mtt)-OH

5-04733 1g Ask for price

Fmoc-Dab(Mtt)-OH

5-04734 5g Ask for price

Fmoc-Dab(Dde)-OH

5-04739 1g Ask for price

Fmoc-Dab(Dde)-OH

5-04740 5g Ask for price

 

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