Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro.

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May 20, 2021 Lieven 0 Comments

Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro.

The aim of this study was to assess the factors, mechanisms and the differences between periodontal ligament (PDL) cells and denta l follicle (DF) progenitor cells towards the osteoblastic/cementoblastic differentiation and to investigate the effects of BMP-7 on developmental (DF) and mature tissue-derived (PDL) cells, respectively. Primary cell culture of PDL cells and DF progenitor cells was performed. Osteogenic differentiation was evaluated using von Kossa, Alizarin Red S and immuno–histo–chemistry staining of osteocalcin.
Gene expression pattern was evaluated via real-time PCR. A series of CD surface marks were tested using flow cytometry and fluorescence-activated cell-sorting analysis was performed. Real-time RT-PCR demonstrated similar gene expression pattern of PDL cells and DF progenitor cells: the expression of OPN and OCN significantly was elevated when incubated with osteogenic components, Runx2 was unaffected, and Osteorix was hardly expressed whether in basic medium or induction medium. In addition, BMP-7 induced osteoblast/cementoblast differentiation of PDLSCs and DF progenitor cells in a dose- and time-dependent manner, as reflected by enhanced Runx2 and (OCN) mRNA transcript expression. BMP-7 triggers PDL cells and DF progenitor cells to differentiate towards an osteoblast/cementoblast phenotype.

 

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Recombinant DNA Fragmentation Factor Subunit Alpha (DFFa)
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DNA Fragmentation Factor 40 kDa Subunit (DFF40) Antibody
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Recombinant Human DNA Fragmentation Factor Subunit Alpha
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Human DNA Fragmentation Factor Subunit Alpha (DFFa) Protein
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Mouse DNA Fragmentation Factor Subunit Alpha (DFFa) Protein
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Human DNA Fragmentation Factor Subunit Alpha (DFFa) Protein
abx167049-100g 100 µg
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Human DNA Fragmentation Factor Subunit Alpha (DFFa) Protein
abx167049-10g 10 µg
EUR 225

Human DNA Fragmentation Factor Subunit Alpha (DFFa) Protein
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EUR 400

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Recombinant Human DNA fragmentation factor subunit beta (DFFB)
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[Analysis on clinical and pathological characteristics of 66 patients with stage IV breast cancer].

OBJECTIVE
To explore the clinical and pathological characteristics of stage IV breast cancer and to analyze their relationship with the morbidity and prognosis.
METHODS
The records of 66 patients presenting from January 2008 to December 2014 with stage IV breast cancer were reviewed. All of the patients were women and the median age was 57.5 (31 to 80) years, accounted for 3.01% (66/2 189) among the breast cancer patients treated in the same period. Statistical methods were used to analyze the correlation between clinical and pathological characteristics such as T-stage, N-stage, immuno–histo–chemistry and the morbidity and prognosis of stage IV breast cancer. The influence of patients characteristics to metastasis were compared by χ(2) test. Kaplan-Meier curves were reported for overall survival (OS), and the Log-rank test was used to compare the difference in groups. Cox proportional models were fitted for multivariate analysis.
RESULTS
The median survival time of stage IV breast cancer was 56.0 months and the 5-year survival rate was 40%. To metastasis, the effects of age, subtypes, histological grade, hormone receptor (HR) and human epidermal growth factor receptor 2 (HER2)had no significant statistics differences. It was concluded that the expression of HER2 (P=0.003) and HR (P=0.001) as well as single metastasis (P=0.029) were the influencing factors of the survival by multivariate Cox regression analysis. Primary tumor R0 surgery group and no surgery group had no significant statistics differences of overall survival and the 5-year survival rate (P=0.102).
CONCLUSIONS
Clinical and pathological characteristics have no effect on metastasis. The expression of HER2 and HR as well as single metastasis play important roles in survival.

Cell lines of human oral squamous-cell carcinomas retaining their differentiated phenotype.

Two cell lines from head-and-neck squamous-cell carcinomas (SCC) have been established and characterized. Cell line R105 was derived from a xenografted SCC of the floor of the mouth and cell line T87/rc from a SCC of the epiglottis. Identification of individual cytokeratins 4, 5, 7, 8, 10, 13, 14, 18 and 19 led to the conclusion that both cell types had squamous characteristics and that keratinization occurred in xenografts. Ultrastructurally, junctional complexes were observed in both cell lines. Characteristic marker chromosomes were found and although both cell lines were derived from male patients, the Y chromosome was missing from all examined cells. The basic biological parameters of both cell lines were modal chromosome numbers of 59 (R105) and 60 (T87/rc), a doubling time of 60 (R105) and 45 hr (T87/rc) and a DNA index of 1.54 (R105) and 1.31 (T87/rc).
The tumorigenicity of the 2 cell lines was proved by the ability to form colonies on a plastic substratum, as well as in a soft agar assay. Furthermore, the cells could produce multi-cellular tumour spheroids, and formed tumour nodules after subcutaneous inoculation into nude mice. The R105 tumour cells appeared to be better differentiated than the T87/rc as observed by histology and immuno(histo)chemistry. Both cell lines appear to retain SCC differentiation after being xenografted into nude mice, cultured for more than 40 passages in vitro and thereafter again xenografted into nude mice.

Tissue engineering in urinary bladder: morphological and functional characterization.

BACKGROUND
In the last years, tissue engineering has attracted lots of researchers, in urology too. This is due to the possibility to use this technique in several pathologies’ therapies, which generally require reconstructive surgical solutions. Our work’s aim is to evaluate morphological and functional aspects of cultivated urothelial and detrusorial tissues, both in “monolayer growth” and on scaffolds, in order to understand the chance of using them in reconstructive surgery.
METHODS
Tissue cultures of detrusorial and urothelial cells have been obtained from animals (pigs, rabbits) and men. The urothelial nature of obtained cells has been demonstrated by traditional histological observation (Hematoxylin – Eosin), by immuno-fluorescence assay (specific for cyto-keratins antibodies), by immuno–histo–chemistry techniques (using specific cyto-keratins 7, 17, and 20 antibodies). Detrusorial tissue has been studied by using antibodies specific for alpha-actin.
RESULTS
Urothelial and smooth muscle cells, when isolated and expanded in vitro, keep the typical characteristics of original tissue, as showed by classical histological observation (H-E), immuno–histo–chemistry and immuno-fluorescence assays. These results were positive both in monolayer colonies and on scaffolds. In vitro results are encouraging and they demonstrate that it is possible to obtain in vitro vesical tissue that could have analogous characteristics to the original organ; even though clinical utilisation of this technique must be more investigated, both in vitro and in vivo.

 

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EUR 79.65

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EUR 79.65

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EUR 79.65

AFP, Prediluted for Immunohistochemistry
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EUR 79.65

AαSM, Prediluted for Immunohistochemistry
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EUR 120.05

Brdu, Prediluted for Immunohistochemistry
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PCNA, Prediluted for Immunohistochemistry
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MUC2, Prediluted for Immunohistochemistry
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S100, Prediluted for Immunohistochemistry
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GFAP, Prediluted for Immunohistochemistry
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A1AT, Prediluted for Immunohistochemistry
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CK 19, Prediluted for Immunohistochemistry
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CK 18, Prediluted for Immunohistochemistry
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HBcAg, Prediluted for Immunohistochemistry
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CK AE1, Prediluted for Immunohistochemistry
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EUR 120.05

CK AE3, Prediluted for Immunohistochemistry
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EUR 120.05

S 100, Prediluted for Immunohistochemistry
MM-1017-04 7.0 ml
EUR 120.05

Lambda, Prediluted for Immunohistochemistry
RP-4021-04 7.0 ml
EUR 79.65

Desmin, Prediluted for Immunohistochemistry
RP-4023-04 7.0 ml
EUR 79.65

CK 8+18, Prediluted for Immunohistochemistry
MM-1007-04 7.0 ml
EUR 120.05

CK 10+13, Prediluted for Immunohistochemistry
MM-1008-04 7.0 ml
EUR 120.05

Insulin Prediluted for Immunohistochemistry
RP-4012-04 7.0 ml
EUR 79.65

PR Immunohistochemistry control tissue array
PM2-PR each
EUR 78
Description: PR Immunohistochemistry control tissue array, including TNM and pathology grade of breast cancer, 11 cases/11 cores

Ferritin, Prediluted for Immunohistochemistry
RP-4001-04 7.0 ml
EUR 79.65

Vimentin, Prediluted for Immunohistochemistry
RP-4002-04 7.0 ml
EUR 79.65

Lysozyme, Prediluted for Immunohistochemistry
RP-4018-04 7.0 ml
EUR 79.65

Calcitonin, Prediluted for Immunohistochemistry
RP-4007-04 7.0 ml
EUR 79.65

Ubiquintin, Prediluted for Immunohistochemistry
RP-4011-04 7.0 ml
EUR 79.65

CK cocktail. Prediluted for Immunohistochemistry
MM-1012-04 7.0 ml
EUR 191.1

Transferrin, Prediluted for Immunohistochemistry
RP-4022-04 7.0 ml
EUR 79.65

Sialosyl Le (a), Prediluted for Immunohistochemistry
MM-1003-04 7.0 ml
EUR 120.05

Synaptophysin, Prediluted for Immunohistochemistry
RP-4008-04 7.0 ml
EUR 79.65

Normal Rabbit Control Serum (for immunohistochemistry)
NRS-IHC 500 μl
EUR 267.84

Myeloperoxidase (MPO), Prediluted for Immunohistochemistry
RP-4025-04 7.0 ml
EUR 79.65

Luteinizing Hormone (LH), Prediluted for Immunohistochemistry
RP-4024-04 7.0 ml
EUR 79.65

Cytokeratin AE1 and AE3, Prediluted for Immunohistochemistry
MM-1022-04 7.0 ml
EUR 136.15

Breast cancer with ER Immunohistochemistry control tissue array
PM2a-ER each
EUR 78
Description: Breast cancer with ER Immunohistochemistry control tissue array, including pathology grade, TNM, clinical stage and IHC results of ER/PR/Her-2/Ki67, 10 cases/10 cores, replacing PM2-ER

OOMB00003-10ML - Blocking Buffer for Immunocytochemistry and Immunohistochemistry
OOMB00003-10ML 10mL
EUR 79

OOMB00006-100ML - 10X Antigen Retrieval Solution pH 6.0 for Immunohistochemistry
OOMB00006-100ML 100mL
EUR 79

OOMB00007-100ML - 10X Antigen Retrieval Solution pH 9.0 for Immunohistochemistry
OOMB00007-100ML 100mL
EUR 79

OOMB00004-10ML - Permeabilization Buffer for Immunocytochemistry and Immunohistochemistry
OOMB00004-10ML 10mL
EUR 79

Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH9.0)
E-IR-R220-10mL 10mL
EUR 325

Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH9.0)
E-IR-R220-3mL 3mL
EUR 125

Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH9.0)
E-IR-R220-each each Ask for price

Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH6.0)
E-IR-R221-10mL 10mL
EUR 325

Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH6.0)
E-IR-R221-3mL 3mL
EUR 125

Super PlusTMHigh Sensitive and Rapid Immunohistochemical Kit (pH6.0)
E-IR-R221-each each Ask for price

DAB Substrate kit
30015-1 KT
EUR 111

DAB Substrate Buffer
ACC500 500 ml
EUR 18.01

DAB Substrate Buffer
ACC999 1000 ml
EUR 24.43

DAB Substrate buffer, 70ml
NB314SB 70 ml
EUR 331.2

DAB Chromogen/Substrate Kit
ACH500 500 Slides
EUR 28.28

DAB Substrate (High Contrast)
ACU250 250 ml
EUR 28.28

DAB Substrate (High Contrast)
ACU500 500 ml
EUR 38.59

DAB Substrate (High Contrast)
ACU999 1000 ml
EUR 55.28

DAB SUBSTRATE BUFFER Reagent
GWB-Q00292 2 x 50ml Ask for price

DAB Chromogen/Substrate Bulk pack
ACK500 530 ml
EUR 56.58

DAB Chromogen/Substrate Bulk pack
ACK999 1060 ml
EUR 101.58

DAB Chromogenic Substrate Kit (Blue)
AR1025 1 kit (for 600-900 assays)
EUR 96

DAB Chromogenic Substrate Kit (Brown)
AR1022 1 kit (for 600-900 assays)
EUR 96

DAB Chromogen/Substrate Kit (High Contrast)
ACT500 500 Slides
EUR 86.15

3,3-Diaminobenzidine (DAB) 5mg Substrate Tabs
CH027 50 Tabs
EUR 212.4

3,3-Diaminobenzidine (DAB) 5mg Substrate Tabs
CH028 100 Tabs
EUR 335

DAB Chromogen/Substrate Bulk Pack (High Contrast)
ACV250 265 ml
EUR 60.07

DAB Chromogen/Substrate Bulk Pack (High Contrast)
ACV500 530 ml
EUR 92.57

DAB Chromogen/Substrate Bulk Pack (High Contrast)
ACV999 1060 ml
EUR 149.13

IHC-Tek DAB Peroxidase Substrate Kit - 120 ml
IW-1600 -
EUR 125

DAB Liquid/Substrate (stable), Background-free; 2-
NB314SBD 70 ml
EUR 295
Description: DAB Liquid/Substrate (stable), Background-free; 2-component system, 70 ml (Large volume)

DAB Liquid/Substrate (stable), Background-free; 2-
NB314SBD-35 35 ml
EUR 225
Description: DAB Liquid/Substrate (stable), Background-free; 2-component system, 35 ml (Small volume)

ImmunoHistozyme
AR-6547-01 15 ml
EUR 47.75

ImmunoHistozyme
AR-6547-02 100 ml
EUR 95.55

ImmunoHistoMount™
AR-6503-01 30 ml
EUR 60.05

ImmunoHistoMount™
AR-6503-02 100 ml
EUR 111.65

ImmunoHistoMount™
AR-6503-03 250 ml
EUR 203.35

ImmunoHistoMount™
AR-6503-05 1000 ml
EUR 556.5

IHC-Tek DAB Chromogen Substrate Single Component 50 ml
IW-1602 -
EUR 85

Black-DAB Substrate/Chromogen System (stable) for
NB319-BB70 70 ml
EUR 389
Description: Black-DAB Substrate/Chromogen System (stable) for IHC staining, 70 ml (Large volume); Ready-To-Use

Black-DAB Substrate/Chromogen System (stable) for
NB319-SBB 30 ml
EUR 289
Description: Black-DAB Substrate/Chromogen System (stable) for IHC staining, 30 ml (Small volume); Ready-To-Use

Human Ras Related C3 Botulinum Toxin Substrate 1 Chemiluminescent Immunoassay Kit
IHURAC1KTC each
EUR 545
Description: Human Ras Related C3 Botulinum Toxin Substrate 1 Chemiluminescent Immunoassay Kit

Rat Fibroblast Growth Factor Receptor Substrate 3 Chemiluminescent Immunoassay Kit
IRTFRS3KTC each
EUR 545
Description: Rat Fibroblast Growth Factor Receptor Substrate 3 Chemiluminescent Immunoassay Kit

Mouse Fibroblast Growth Factor Receptor Substrate 2 Chemiluminescent Immunoassay Kit
IMSFRS2KTC each
EUR 545
Description: Mouse Fibroblast Growth Factor Receptor Substrate 2 Chemiluminescent Immunoassay Kit

TMB Substrate
abx293006-100ml 100 ml
EUR 326.4

TMB Substrate
abx098953-9ml 9 ml
EUR 117.6

TMB Substrate
85R-117 125 ml
EUR 171
Description: High sensitivity TMB Substrate ready to use in immunoassays

TMB Substrate
85R-118 60 ml
EUR 169
Description: High sensitivity TMB Substrate for use in ELISA

TMB Substrate
85R-122 60 ml
EUR 247
Description: High sensitivity TMB Substrate especially designed for the microArray applications

PKG Substrate
HY-P1561 1mg
EUR 211.2

PKG Substrate
TP1787-10mg 10mg Ask for price
Description: PKG Substrate

PKG Substrate
TP1787-1g 1g Ask for price
Description: PKG Substrate

PKG Substrate
TP1787-1mg 1mg Ask for price
Description: PKG Substrate

PKG Substrate
TP1787-50mg 50mg Ask for price
Description: PKG Substrate

PKG Substrate
TP1787-5mg 5mg Ask for price
Description: PKG Substrate

TMB Substrate
abx299722-100g 100 µg Ask for price

TMB Substrate
abx299722-20g 20 µg
EUR 450

TMB Substrate
abx299722-50g 50 µg Ask for price

CERK Substrate
40618 150 rxns.
EUR 225
Description: Substrate for ceramide kinase (CERK), optimized for use with β-Octylglucoside mixed micellar assay.

CERK Substrate
40619 300 rxns.
EUR 415
Description: Substrate for ceramide kinase (CERK), optimized for use with β-Octylglucoside mixed micellar assay.

PNPP Substrate
F008-22 22 ml
EUR 216
Description: PNPP Substrate by Cygnus Technologies is available in Europe via Gentaur.

ABTS Substrate
42-AB08S 500 ml
EUR 133
Description: High sensitivity HRP microwell substrate (containing ABTS)

Cdk5 Substrate
T37207-10mg 10mg Ask for price
Description: Cdk5 Substrate

Cdk5 Substrate
T37207-1g 1g Ask for price
Description: Cdk5 Substrate

Cdk5 Substrate
T37207-1mg 1mg Ask for price
Description: Cdk5 Substrate

Cdk5 Substrate
T37207-50mg 50mg Ask for price
Description: Cdk5 Substrate

Cdk5 Substrate
T37207-5mg 5mg Ask for price
Description: Cdk5 Substrate

LSD1 Substrate
GWB-E62684 500µl Ask for price

CERK Substrate
GWB-PS34FD 150rxns. Ask for price

CERK Substrate
GWB-PS66AB 300rxns. Ask for price

MARK Substrate
HY-P1583 10mg
EUR 459.6

NQO1 substrate
T13799-10mg 10mg Ask for price
Description: NQO1 substrate

NQO1 substrate
T13799-1g 1g Ask for price
Description: NQO1 substrate

NQO1 substrate
T13799-1mg 1mg Ask for price
Description: NQO1 substrate

NQO1 substrate
T13799-50mg 50mg Ask for price
Description: NQO1 substrate

NQO1 substrate
T13799-5mg 5mg Ask for price
Description: NQO1 substrate

pNPP Substrate
X156-11ML 11ML
EUR 218

MARK Substrate
TP1841-10mg 10mg Ask for price
Description: MARK Substrate

MARK Substrate
TP1841-1g 1g Ask for price
Description: MARK Substrate

MARK Substrate
TP1841-1mg 1mg Ask for price
Description: MARK Substrate

MARK Substrate
TP1841-50mg 50mg Ask for price
Description: MARK Substrate

MARK Substrate
TP1841-5mg 5mg Ask for price
Description: MARK Substrate

PLPro Substrate
79997 1 mg
EUR 350
Description: The PLPro (Papain-Like Protease) substrate is a small 5-mer fluorogenic peptide (Z- RLRGG-AMC) used to measure the activity of SARS family PLPro proteases. Release of the C-terminal AMC dye (7-amido-4-methylcoumarin ) by proteolysis generates fluorescence, which can be monitored at an excitation wavelength of 345 nm and an emission wavelength of 445 nm.Conditions optimized for use with: Papain-like Protease (SARS-CoV-2) Assay Kit: Protease Activity (BPS Bioscience #79995). The peptide corresponds to a short sequence of ubiquitin and may also be used as substrate of USP5 (isopeptidase T) and ubiquitin C-terminal hydrolases (UCH family, for example UCHL1).

Renin Substrate
CP7218-1mg 1.0mg
EUR 90

Renin Substrate
CP7218-5mg 5.0mg
EUR 237

DiFMUP Substrate
79769 50 µl
EUR 95
Description: The fluorogenic SHP-2 substrate, DiFMUP, is added to the mixture of SHP-2 (BPS Bioscience #79018) and SHP-2 Activating Peptide (BPS Bioscience #79319). DiFMU fluorophore is released and can then be measured using a fluorescence reader.

Lipase substrate
L246860 100mg
EUR 11200
Description: 195833-46-6

Lipase Substrate
T39383-10mg 10mg Ask for price
Description: Lipase Substrate

Lipase Substrate
T39383-1g 1g Ask for price
Description: Lipase Substrate

Lipase Substrate
T39383-1mg 1mg Ask for price
Description: Lipase Substrate

Lipase Substrate
T39383-50mg 50mg Ask for price
Description: Lipase Substrate

Lipase Substrate
T39383-5mg 5mg Ask for price
Description: Lipase Substrate

H3K4me2 Substrate
50158 500 µl
EUR 230
Description: Histone H3 peptide di-methylated at Lysine 4, substrate for LSD1

MARCKS Substrate (151-175)
5-01505 4 x 1mg Ask for price

Endotoxin Substrate
4007683.005 50 mg
EUR 196.67

Endotoxin Substrate
4007683.025 250 mg
EUR 786.45

Endotoxin Substrate
L-1195.0050 50.0mg
EUR 340.8
Description: Sum Formula: C25H40N8O7; CAS# [68223-96-1] net

Endotoxin Substrate
L-1195.0250 250.0mg
EUR 1255.2
Description: Sum Formula: C25H40N8O7; CAS# [68223-96-1] net

RSK Substrate, S6 (231-239)
5-01888 4 x 5mg Ask for price

Nitrocefin substrate
42R-1003 5 mg
EUR 472
Description: Nitrocefin substrate for use in the research laboratory

AEC Substrate Kit
AR1020 3mL X 2 (for 600-900 assays)
EUR 85.2

Adam 17 Substrate
CP7216-1mg 1.0mg
EUR 90

Adam 17 Substrate
CP7216-5mg 5.0mg
EUR 237

BACE Substrate II
CP7221-1mg 1.0mg
EUR 90

BACE Substrate II
CP7221-5mg 5.0mg
EUR 237

Calcineurin Substrate
5-00898 4 x 1mg Ask for price

Phosphatase Substrate
5-01759 4 x 1mg Ask for price

Calcineurin Substrate
SP-51014-1 1 mg
EUR 205.2

Calcineurin Substrate
H-2084.0001 1.0mg
EUR 400.8
Description: Sum Formula: C92H150N28O29; CAS# [113873-67-9] net

Calcineurin Substrate
H-2084.0005 5.0mg
EUR 1488
Description: Sum Formula: C92H150N28O29; CAS# [113873-67-9] net

Calcineurin Substrate
GWB-672C81 1 mg Ask for price

 

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