GelRedTM Nucleic Acid Gel Stain, 1

July 6, 2021 0 Comments


GelRed is an ultra-sensitive, extremely stable, and environmentally safe fluorescent nucleic acid dye designed to replace highly toxic ethidium bromide (EtBr) for staining dsDNA, ssDNA, or RNA in agarose gels or polyacrylamide gels.

  • Safer than EtBr – non-mutagenic and non-hazardous to remove
  • Much more sensitive than EtBr and SYBR® Safe
  • Stable at room temperature and microwave safe.
  • Pre-cast or post-electrophoresis simple gel staining, no discoloration
  • Replaces EtBr with no changes to equipment or optical settings
  • Supports gel purification, restriction digestion, sequencing, and subsequent cloning

GelRed: a superior alternative to EtBr

GelRed is much more sensitive than EtBr and can be used to stain dsDNA, ssDNA, or RNA in agarose gels by pre-cast or post-gel staining without discoloration. GelRed can also be used to stain polyacrylamide gels by post gel staining. GelRed is also compatible with subsequent DNA manipulations such as restriction digestion, sequencing, and cloning. GelRed and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed without changing your existing imaging system. For detailed usage protocols, download the GelRed product information sheet.

Non-mutagenic and safer for the environment

A series of safety tests have confirmed that GelRed is not cytotoxic, non-mutagenic, and is not dangerous at concentrations well above the working concentrations used in gel staining. As a result, Workforce GelRed can be safely disposed of down the drain or in common trash, providing convenience and reducing the cost of waste disposal. For detailed test results, you can download the GelRed / GelGreen Safety Report.

How safe is your gel stain?

Many of the so-called “safe” DNA stains such as SYBR Safe, Midori Green, GreenSafe, SafeView ™, and RedSafe ™ not only have low sensitivity, but also easily penetrate living cells to bind to DNA, and some are cytotoxic. Unlike these dyes, GelRed is impermeable to the cell membrane, so it cannot enter living cells to interact with their DNA. Please see our Gel Stain Comparison Brochure or Gel Stain Comparison White Paper for more details.

Choose the right tint for your application

GelRed 3X in Water (catalog number 41001) is ready to use for post electrophoresis gel staining and is supplied in a 4L Cubitainer. Biotium also offers GelRed 10,000X in water (catalog number 41003) and GelRed 10,000X in DMSO (catalog number 41002). GelRed in water is a newer, safer formula and our recommended format. We continue to offer GelRed in DMSO for established users who do not wish to alter their protocols. We also offer GelRed Agarose and GelRed Pre stain Plus 6X Loading Dye.

GelRed and its uses are covered by issued or pending the US and international patents. GelRed and EvaGreen are registered trademarks of Biotium, Inc. SafeView is a trademark of Applied Biological Materials; RedSafe is a trademark of iNtRON Biotechnology. SYBR is a registered trademark of Thermo Fisher Scientific. Cubitainer is a registered trademark of Hedwin Corporation.

Leave a Reply

Your email address will not be published. Required fields are marked *