TRITC conjugated Ulex Europaeus Le

July 6, 2021 0 Comments

Properties

Form: lyophilized powder

Power: <10 μg / ml agglutination activity

Composition: Protein, ~ 10% Lowry

Labeling extension: 4-8 mol of TRITC per mol of lectin

Conjugate: Conjugate TRITC

Storage temperature: −20 ° C

Biochemical / Physiological Actions

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N, N’-diacetylchitobiose. The mole. Weight of UEA Initially it was found to be 170,000 daltons. Later reports indicate that UEA I can form aggregates at neutral and basic pH and that the mole is correct. weight is 68,000 daltons.

Packaging

Package size based on protein content.

Link

Conjugates are prepared from affinity-purified lectin.

Physical form

Contains buffer salts of potassium phosphate and sodium chloride

Analysis note

Agglutination activity is expressed in µg / ml and is determined from serial dilutions in phosphate-buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour of incubation at 25 ° C.

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